Vineet Augustine

STAR★Methods REAGENT or RESOURCESOURCEIDENTIFIER Antibodies Anti-IL1β antibody Invivolab BE0246; RRID: AB_2687727 Chicken anti-TH Aves Lab TYH-0020; RRID: AB_10013440 Mouse anti-VEGF Santa Cruz Biotech sc7269; RRID: AB_628430 Rabbit anti-Ki67 Abcam ab16667; RRID: AB_302459 Mouse anti-TNFα Abcam ab1793; RRID: AB_302615 Rabbit anti-CD31 Abcam ab28364; RRID: AB_726362 Rat anti-mCherry Invitrogen M11217; RRID: AB_2536611 Rabbit anti-GFP Abcam ab290; RRID: AB_303395 Mouse anti-cFOS Santa Cruz...

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Highlights • Thirst neurons in the brain receive temporally distinct multiple satiation signals • Liquid gulping and gut osmolality signals transmit thirst satiation • Specific GABAergic neurons in the subfornical organ (SFO) mediate satiation signals from the gut • Drinking-induced dopamine release is independent of thirst satiation signals Summary For thirsty animals, fluid intake provides both satiation and pleasure of drinking. How the brain processes these factors is currently unknown.