vipin kumar

Abstract Improved methods to identify therapeutically relevant tumor neoantigens and their cognate T cells would aid the development of precision medicines for cancer. Here, we developed Slide-GoTags, a droplet-based single-nucleus spatial transcriptomics approach that characterizes neoantigen-specific immunity by integrating targeted transcript genotyping and T cell receptor (TCR) sequencing with single-nucleus RNA sequencing from the same slice of frozen tissue.

Abstract The mammalian cerebral cortex comprises a complex neuronal network that maintains a precise balance between excitatory pyramidal neurons and inhibitory interneurons. Accumulating evidence indicates that specific interneuron subtypes form stereotyped microcircuits with distinct pyramidal neuron classes1,2,3. Here we show that pyramidal neurons have an active role in this process by promoting the survival and terminal differentiation of their associated interneuron subtypes.

Abstract Spatial transcriptomics enables gene expression mapping within tissues but is often limited by imaging constraints. We present an imaging-free approach that reconstructs spatial barcode locations using molecular diffusion and dimensionality reduction. Validated against ground truth imaging, our method achieves high fidelity and scales to centimeter-sized tissues.