Xiju Xia

Keywords presynaptic Ca2+ GRAB sensor peptidergic neurotransmission circadian and sleep rhythms secretory granule fluorogen-activating protein exocytosis Results and discussion sLNv neurons, via their release of PDF, are important morning clock neurons.1,4 Thus, for understanding the circadian clock circuit, it is of interest to determine when activity-dependent synaptic neuropeptide release from sLNv terminals occurs during the day.

Abstract The co-existence and co-transmission of neuropeptides and small molecule neurotransmitters in the same neuron is a fundamental aspect of almost all neurons across various species. However, the differences regarding their in vivo spatiotemporal dynamics and underlying molecular regulation remain poorly understood. Here, we developed a GPCR-activation-based (GRAB) sensor for detecting short neuropeptide F (sNPF) with high sensitivity and spatiotemporal resolution.

1State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China 2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China 3Yuanpei College, Peking University, Beijing 100871, China 4Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China 5Peking University–Tsinghua University–National Institute of Biological Sciences Joint Graduate Program, Academy for Advanced...