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Research Article SYNTHETIC BIOLOGY INTRODUCTION Renewable one-carbon (C1) substrates, particularly methanol and formate, present an appealing alternative to traditional sugar-based substances for biochemical production (1, 2). As methanol and formate can be efficiently derived from CO2 through advanced electrocatalysis methods, their utilization in microbial fermentation heralds a future of sustainable and carbon-neutral biochemical production (3, 4).
Glossary C2+ chemicals organic compounds that contain two or more carbon atoms. Calvin–Benson–Bassham (CBB) cycle a series of redox reactions that takes place in the stroma of chloroplasts in photosynthetic organisms. Carbon-neutral/carbon-negative bioproduction a bioprocess of net zero/negative carbon dioxide emissions. Crabtree-negative yeast yeast that produces only biomass and carbon dioxide, instead of ethanol, during aerobic fermentation.
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