ABSTRACT Abalone peptides are promising bioactive compounds with potential health benefits. This study developed an efficient, mild enzymatic process using alkaline protease to extract these peptides. Structural characterization showed a low average molecular weight (~720 Da), with over 70% under 1000 Da, and a predominantly random coil conformation. Analysis revealed high contents of glycine, alanine, glutamate, and aspartate, with ~33% hydrophobic residues and Pro-rich sequences.