Yasuha Kinugasa

CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest. Supporting Information Filename Description gtc13180-sup-0001-Figures.docxWord 2007 document , 5.9 MB Figure S1. Auxin-inducible degradation of mAID-tagged SMC6 in HeLa cells. (A) Immunoblots verifying CRISPR/Cas9 mediated mAIDmClover tagging to endogenous SMC6 gene and showing degradation of SMC6 after 24 h of IAA treatment. SMC6 remained undegraded in HeLa cells expressing only the OsTIR1 (parental cells).

Abstract Cancer cells collectively form a large-scale structure for their growth. In this paper, we report that HeLa cells, epithelial-like human cervical cancer cells, aggressively migrate on Matrigel and form a large-scale structure in a cell-density dependent manner. To explain the experimental results, we develop a simple model where cells interact and migrate using the two fundamentally different types of force, remote and contact forces, and show how cells form a large-scale structure.