Ying-Hui Ko

Keywords nucleocytoplasmic transport NPC NLS importin 7 importin β β-karyopherins cytokine signaling The abbreviations used are IPO7 (importin 7) NLS (nuclear localization signal) cNLS (classical NLS) NTS (nuclear translocation signal) IBB (Importin β binding) BIB (beta-like import receptor binding domain) NES (nuclear export signal) FG-nup (Phenylalanine-Glycine nucleoporins) RanBP (Ran-binding protein) ROS (reactive oxygen species) cryo-EM (cryogenic electron microscopy) HPV (human...

Abstract Iron is essential for growth of Mycobacterium tuberculosis (Mtb), but most iron in the human body is stored in heme within hemoglobin. Here, we demonstrate that the substrate-binding protein DppA of the inner membrane Dpp transporter is required for heme and hemoglobin utilization by Mtb. The 1.27 Å crystal structure of DppA shows a tetrapeptide bound in the protein core and a large solvent-exposed crevice for heme binding.

A subgroup of breast cancers has several metabolic compartments. The mechanisms by which metabolic compartmentalization develop in tumors are poorly characterized. TP53 inducible glycolysis and apoptosis regulator (TIGAR) is a bisphosphatase that reduces glycolysis and is highly expressed in carcinoma cells in the majority of human breast cancers. Hence we set out to determine the effects of TIGAR expression on breast carcinoma and fibroblast glycolytic phenotype and tumor growth.