Ying Sin Koo

Abstract Biocatalysis provides a sustainable approach for highly selective chemical transformations, however the complex, non-additive nature of enzyme fitness landscapes remains a significant barrier hindering the predictive design of optimized enzymes. Here, we document a curated dataset of kinetic colorimetric activity measurements of galactose oxidase variants for biocatalytic oxidation of a secondary alcohol, 1-phenylbutan-1-ol.

Introduction Click to copy section linkSection link copied! Enzymatic reactions are favored for their exquisite regio- and stereoselectivity, operation under mild reaction conditions, and use of environmentally benign reagents that enable sustainable chemical transformations. (1−3) Their high selectivities minimize byproduct formation, reduce purification costs, increase product yields, and enable multistep biocatalytic cascades in a single pot.

Analytical Chemistry Cite this: Anal. Chem. 2025, XXXX, XXX Click to copy citationCitation copied! . This publication is licensed under CC-BY-NC-ND 4.0 . You are free to share (copy and redistribute) this article in any medium or format within the parameters below: Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Non-Commercial (NC): Only non-commercial uses of the work are permitted.