Yingjin Yuan

Abstract Enzyme catalysis has become a cornerstone of modern synthesis, offering sustainable routes to structurally complex molecules. However, the rugged fitness landscape and limited model capacity to learn from sparse data constrain the development of biocatalysts. Here, we present Rank-guided Exploration for Automated enzyme reProgramming (REAP), a closed-loop platform that combines artificial intelligence with robotic experimentation to accelerate enzyme engineering.

Abstract Deoxyribonucleic acid provides unmatched information density and longevity for data storage, yet its easy amplification by polymerase chain reaction enables unauthorized replication at negligible cost. We introduce ZAT-DNA, which encodes information in patterns of canonical adenine and noncanonical 2-aminoadenine.

Abstract Oligonucleotide multiplicity is an inherent property of current DNA synthesis technology. Composite letter DNA storage exploits this property to improve logical density and reduce costs. However, letter indistinguishability and high molecular diversity pose challenges for reliable recovery. Here, we formulate a composite letter constellation model, named DNA diamond, consisting of 15 decomposable points.