Yingjun Guan

Abstract Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disorder characterized by progressive loss of motor neurons (MNs). N6-methyladenosine (m6A) is the most abundant mRNA modification, yet its role in ALS MNs degeneration remains poorly understood. In this study we observed a significant decrease in global m6A levels in the spinal cords of hSOD1G93A transgenic ALS mouse model and hSOD1G93A NSC34 cells, accompanied by reduced expression of the methyltransferase METTL3.

4.1. Inter-Level Transition Test To validate the stability of the parallel triangular configuration structure constructed based on the theoretical node displacement description using the absolute nodal coordinate formulation (ANCF), a 1:1 scale experimental prototype was designed and fabricated. The material selection and boundary conditions were set to be consistent with those used in the theoretical and simulation stages. The experimental setup can be referred to in Figure 20.

MicroRNAs (miRNAs) are suspected to be a contributing factor in amyotrophic lateral sclerosis (ALS). Here, we assess the altered expression of miRNAs and the effects of miR‐124 in astrocytic differentiation in neural stem cells of ALS transgenic mice. Differentially expressed miRNA‐positive cells (including miR‐124, miR‐181a, miR‐22, miR‐26b, miR‐34a, miR‐146a, miR‐219, miR‐21, miR‐200a, and miR‐320) were detected by in situ hybridization and qRT‐PCR in the spinal cord and the brainstem.