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Ultraphotostable Phosphorescent Nanosensors for Sensing the Lysosomal pH at the Single-Cell Level over Long Durations
Download Hi-Res ImageDownload to MS-PowerPointCite This:Anal. Chem. 2024, XXXX, XXX Terms & Conditions Most electronic Supporting Information files are available without a subscription to ACS Web Editions. Such files may be downloaded by article for research use (if there is a public use license linked to the relevant article, that license may permit other uses).
Identification of Three Sequence Motifs in the Transcription Termination Factor Sen1 that Mediate Direct Interactions with Nrd1
Highlights • Three Nrd1 interaction motifs (NIMs) are identified in C-terminal segment of Sen1 • The NIMs are bound in the same pocket as the Pol II CTD phosphopeptide • Removal of all three NIMs abolishes Nrd1-Nab3-Sen1 complex formation • Removal of all three NIMs produces ncRNA termination defects Summary The Nrd1-Nab3-Sen1 (NNS) complex carries out the transcription termination of non-coding RNAs (ncRNAs) by RNA polymerase II (Pol II) in yeast, although the detailed interactions among its...
A background-subtraction strategy leads to ratiometric sensing of oxygen without recalibration
Luminescence-quenching based optical oxygen sensors have wide applications in many fields, which have already replaced almost 40 % of commercial market share dominated previously by Clark oxygen electrode. The majority of optical oxygen sensors are based on lifetime measurement, which are precise, but are relatively expensive, and require high-speed electronics and detecting circuits.
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