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Yingxiu Cao

As seen in: Cell Press
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Articles

Genome-scale CRISPRi and base-editing libraries for genetic decoding and strain engineering in Shewanella

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By Yaru Chen, Qiyang Duan, Lin Wang, Qianxi He, Lixia Fang, Lin Xiao, Yingxiu Cao
| Cell Press Verified
Keywords CRISPR-based library electroactive microorganism genome-scale library substrate spectrum Get full text access Log in, subscribe or purchase for full access. References 1. Przybyla, L. ∙ Gilbert, L.A. A new era in functional genomics screens Nat. Rev. Genet. 2022; 23:89-103 2. Cheng, Z.H. ... Repurposing CRISPR RNA-guided integrases system for one-step, efficient genomic integration of ultra-long DNA sequences Nucleic Acids Res. 2022; 50:7739-7750 3. Wang, T. ...
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Electricity-driven 7α-Hydroxylation of Steroid Catalyzed by Cytochrome P450 Monooxygenase in Engineered Yeast

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By Feng LI, Yao Tian, Ziyin Zhang, Yingxiu Cao
| Royal Society of Chemistry Verified
Electricity-driven 7α-Hydroxylation of Steroid Catalyzed by Cytochrome P450 Monooxygenase in Engineered Yeast NAD(P)H are the cofactors of the cytochrome P450 monooxygenase for the catalytic hydroxylation of steroids, which is notoriously challengeable to traditional synthetic chemistry due to the requirement of specificity and chirality. However, undersupply of NAD(P)H is a crucial bottleneck in restricting the conversion rate of P450-catalyzed steroidal hydroxylation.
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