Yonglun Luo

Abstract The molecular characterization of chicken cells provides insight into avian physiology and vertebrate evolution. Here we present a single-cell RNA-sequencing (scRNA-seq) atlas of the chicken, encompassing 1.57 million cells across 157 cell types from 36 tissues from 16 chickens, as well as spatial transcriptomes from 1 chicken embryo.

Abstract CRISPR-derived base editors (BE) enable precise single nucleotide substitution without introducing double-stranded DNA breaks. Apart from the base editing enzymes, efficient base editing strongly depends on both the CRISPR guide RNA (gRNA) efficiency and the edited position. Here, we show that the accuracy of BE gRNA design can be significantly improved by generating more data and by introducing deep neural networks trained on multiple different datasets simultaneously.

Abstract Systematic characterization of the molecular states of cells in livestock tissues is essential for understanding the cellular and genetic mechanisms underlying economically and ecologically important physiological traits.