Yukihisa Hayashida

Abstract Intracellular signaling dynamics are often obscured by the spatial and temporal limitations of cell size. Here, we developed a method to enlarge Dictyostelium discoideum cells by partial cytokinesis inhibition, generating multinucleated yet functional giant cells. These cells retained chemotactic signaling, polarity, and motility, enabling high-resolution live-cell imaging.

Abstract Intracellular signaling dynamics are often obscured by the spatial and temporal limitations of cell size. Here, we developed a method to enlarge Dictyostelium discoideum cells by partial cytokinesis inhibition, generating multinucleated yet functional giant cells. These cells retained chemotactic signaling, polarity, and motility, enabling high-resolution live-cell imaging.

Get full text access Log in, subscribe or purchase for full access. References 1. Cooper, S. Control and maintenance of mammalian cell size BMC Cell Biol. 2004; 5:35 2. Chien, A.C. ∙ Hill, N.S. ∙ Levin, P.A. Cell size control in bacteria Curr. Biol. 2012; 22:R340-R349 3. Turner, J.J. ∙ Ewald, J.C. ∙ Skotheim, J.M. Cell size control in yeast Curr. Biol. 2012; 22:R350-R359 4. Wright, J. ∙ Schneider, B.L. Cell size control is sirtuin(ly) exciting Mol. Syst. Biol. 2013; 9:706 5. Kiyomitsu, T.