Zarko Manojlovic

Loading metrics In Fig 3B, the Liver 1 and Liver 2 panels for FK506 were erroneously duplicated. The Liver 1 image is correct. An updated version of Fig 3 is provided here in which the Liver 2 FK506 panel has been replaced. Underlying quantification data supporting Fig 3C from recent replicate analyses of the samples used in the original experiments are available in S2, S3, S4 and S5 Files. The original individual-level quantitative data from which Fig 3C were generated are no longer available.

Abstract Common mitochondrial DNA (mtDNA) deletions are large structural variants in the mitochondrial genome that accumulate in metabolically active tissues with age and have been investigated in various diseases. We applied the Splice-Break2 pipeline (designed for high-throughput quantification of mtDNA deletions) to human RNA-Seq datasets and describe the methodological considerations for evaluating common deletions in bulk, single-cell, and spatial transcriptomics datasets.

Abstract Accurate transcription is required for the faithful expression of genetic information. However, relatively little is known about the molecular mechanisms that control the fidelity of transcription, or the conservation of these mechanisms across the tree of life.