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Cardiovascular baroreflex circuit moonlights in sleep control
Method details Virus preparation AAV2-EF1α-DIO-ChR2-eYFP, AAV2-EF1α-DIO-iC++-eYFP, AAV2-EF1α-DIO-eYFP, AAV2-EF1α-DIO-hM3D(Gq)-mCherry, AAV2-EF1α-DIO-mCherry, AAV2-EF1α-DIO-taCasp3-TEVp were obtained from the University of North Carolina (UNC) vector core. AAV2-hSyn-DIO-hM3D(Gq)-mCherry, AAV2-hSyn-DIO-mCherry, AAV9-CAG-FLEX-GCaMP6s-WPRE.SV40 were obtained from Addgene.
Control of Non-REM Sleep by Midbrain Neurotensinergic Neurons
Alföldi P. Rubicsek G. Cserni G. Obál Jr., F. Brain and core temperatures and peripheral vasomotion during sleep and wakefulness at various ambient temperatures in the rat. Pflugers Arch. 1990; 417: 336-341 Anaclet C. Ferrari L. Arrigoni E. Bass C.E. Saper C.B. Lu J. Fuller P.M. The GABAergic parafacial zone is a medullary slow wave sleep-promoting center. Nat. Neurosci. 2014; 17: 1217-1224 Anikeeva P. Andalman A.S. Witten I. Warden M. Goshen I. Grosenick L. Gunaydin L.A. Frank L.M. Deisseroth K.
Sleep Regulation by Neurotensinergic Neurons in a Thalamo-Amygdala Circuit
Highlights • A three-step approach was used for whole-brain screening for sleep-promoting neurons • NTS-positive GABAergic CeA neurons widely inhibit wake neurons and promote NREM sleep • NTS-positive glutamatergic pTh neurons excite CeA NTS neurons and promote NREM sleep • CeA and pTh neurons require both NTS and GABA and glutamate transmission to promote sleep Summary A crucial step in understanding the sleep-control mechanism is to identify sleep neurons.
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