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Accelerating Medicin

As seen in: Cell Reports
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By Nida Meednu, Javier Rangel-Moreno, fan zhang, Katherine Escalera-Rivera, Elisa Corsiero, Edward F. DiCarlo, Susan Goodman, Laura T. Donlin, Michele Bombardieri, Costantino Pitzalis, Dana E. Orange, Katherine Escalera-Rivera, Edoardo Prediletto, Soumya Raychauduri, Accelerating Medicin
| Cell Reports Verified
NR4A+ synovial B cells accrue SHM To define the Ig repertoire in single B cells, we performed V(D)J sequencing on the same sorted CD19+ cells used for single-cell RNA sequencing (scRNA-seq), allowing a joint analysis of transcriptional profile and immune repertoire in the same cell (Figures 1 and S2). Similar to previously published data, all three naive clusters exhibited very low SHM frequencies, while memory and PC clusters showed the highest mutation rates ( ; ; ) ( Figures 3A and S2F).
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