Agavni Mesropian

ArticleOnline now102717Open access Affiliations & Notes 1Hepatology Laboratory, Solid Tumors Program, CIMA, CCUN, University of Navarra, Pamplona, Spain 2CIBERehd, Instituto de Salud Carlos III, Madrid, Spain 3RNA Biology and Therapy Program, CIMA, CCUN, University of Navarra, Pamplona, Spain 4Instituto de Investigaciones Sanitarias de Navarra IdiSNA, Pamplona, Spain 5Liver Cancer Translational Research Group, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital...

Keywords cancer hepatocellular carcinoma early carcinogenesis tumor heterogeneity malignant transition copy number alteration spatial transcriptomics Immune evasion evolutionary trajectories response to immune therapies Introduction The transition from premalignant lesions to early cancers represents a critical stage in carcinogenesis.

Abstract CTNNB1, the gene encoding β-catenin, is a frequent target for oncogenic mutations activating the canonical Wnt signaling pathway, typically through missense mutations within a degron hotspot motif in exon 3. Here, we combine saturation genome editing with a fluorescent reporter assay to quantify signaling phenotypes for all 342 possible missense mutations in the mutation hotspot.