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Articles by Anna Buchman
Precision Guided Sterile Males Suppress Populations of an Invasive Crop Pest
GEN BiotechnologyVol. 1, No. 4 Research ArticlesFree Access Abstract The Drosophila suzukii invasion of western countries has created an immense agricultural and economic threat to crop production. Despite many attempts to suppress its population, D. suzukii continues to destroy soft-flesh fruits. Precision guided sterile insect technique (pgSIT) utilizes the accuracy of programmable CRISPR gene targeting to generate sterilized males that can be deployed to suppress populations.
Reply to: Assessing the efficiency of Verily’s automated process for production and release of male Wolbachia-infected mosquitoes - Nature Biotechnology
replying to: Bouyer et al. Nature Biotechnology https://doi.org/10.1038/s41587-022-01324-z This is a preview of subscription content Access options Subscribe to Nature+ Get immediate online access to the entire Nature family of 50+ journals Subscribe to Journal Get full journal access for 1 year $99.00 only $8.25 per issue All prices are NET prices. VAT will be added later in the checkout. Tax calculation will be finalised during checkout.
Genetically Encoded CRISPR Components Yield Efficient Gene Editing in the Invasive Pest Drosophila suzukii
Introduction Drosophila suzukii (Matsumura, 1931, Diptera: Drosophilidae), commonly known as spotted wing drosophila, is a significant crop pest1–3 of many soft-skinned fruits that has recently invaded much of the world.4–6 Unlike other Drosophila fly species that infest overripe or rotting fruits, D. suzukii targets ripening fruits.4,6,7D.
Programmable RNA Targeting Using CasRx in Flies
Introduction The development of CRISPR as a programmable genome engineering tool has revolutionized the life sciences by providing transformative applications for both medicine and biotechnology.1 While much of the recent focus has been on exploiting CRISPR technologies to target DNA, recent findings that certain CRISPR systems can also be programmed to target RNA have suggested new possibilities for CRISPR technologies in transcriptome engineering.2–4 For example, one recent advancement was...
Engineered resistance to Zika virus in transgenic Aedes aegypti expressing a polycistronic cluster of synthetic small RNAs
Significance Here, we describe the generation of Aedes aegypti mosquitoes that are engineered to be resistant to Zika virus (ZIKV) transmission. Our results demonstrate that engineered mosquitoes express a polycistronic cluster of synthetic small RNAs designed to target the ZIKV genome. As a result, homozygous mosquitoes were refractory to ZIKV infection, and therefore could not transmit the virus.
Police: Woman attacks responders with cinder block, rocks and a squeegee
By Claire Morgan and Anna Buchman The Sacramento Bee REDDING, Calif. — Emergency crews responding to report of an unresponsive female found themselves under attack with a cinder block, rocks and a squeegee, Redding police said Thursday. Fire department and ambulance responders arrived at 9:23 p.m. Wednesday at the Clear Creek Market on Westside Road to provide aid to Violeta Gallegos of Redding, Police Department Sgt. Brian Cole said. However, Gallegos, 35, reportedly grew combative.
Police: Woman attacks responders with cinder block, rocks, squeegee
By Claire Morgan and Anna Buchman The Sacramento Bee REDDING, Calif. — Emergency crews responding to report of an unresponsive female found themselves under attack with a cinder block, rocks and a squeegee, Redding police said Thursday. Fire department and ambulance responders arrived at 9:23 p.m. Wednesday at the Clear Creek Market on Westside Road to provide aid to Violeta Gallegos of Redding, Police Department Sgt. Brian Cole said. However, Gallegos, 35, reportedly grew combative.
Engineered resistance to Zika virus in transgenic Ae. aegypti expressing a polycistronic cluster of synthetic miRNAs
Engineered resistance to Zika virus in transgenic Ae. aegypti expressing a polycistronic cluster of synthetic miRNAs Anna Buchman, Stephanie Gamez, Ming Li, Igor Antoshechkin, hin-Hang Lee, Shin-Wei Wang, Chun-Hong Chen, Melissa J Klein, Jean-Bernard Duchmemin, Prasad N. Paradkar, Omar S Akbari bioRxiv 344697; doi: https://doi.org/10.1101/344697
Synthetically engineered Medea gene drive system in the worldwide crop pest Drosophila suzukii
SignificanceHere we describe a fully functional gene drive system constructed in a major worldwide crop pest, Drosophila suzukii. This system is composed of a synthetic Medea drive with a maternal miRNA “toxin” and a zygotic “antidote,” and we demonstrate that it can bias inheritance with 100% efficiency and can persist in a population given high release frequencies. We discuss how such a system may be used to suppress D.
Synthetically Engineered Medea Gene Drive System in the Worldwide Crop Pest, D. suzukii
Synthetically Engineered Medea Gene Drive System in the Worldwide Crop Pest, D. suzukii Anna Buchman, John Marshall, Dennis Ostrovski, Ting Yang, Omar S Akbari bioRxiv 162255; doi: https://doi.org/10.1101/162255
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