Arjun Raj

Abstract Cellular responses to environmental stimuli are typically thought to be governed by genetically encoded programs. We demonstrate that cancer cells can form and maintain cellular memories when becoming drug resistant, exhibiting characteristics of cellular learning that are dependent on the transcription factor AP-1. We show that cells exposed to a low dose of therapy adapt to become resistant to a high dose, demonstrating that cells can adapt during this time of stress.

Abstract Imaging-based spatial transcriptomics methods allow for the measurement of spatial determinants of cellular phenotypes but are incompatible with random barcode-based clone-tracing methods, preventing the simultaneous detection of clonal and spatial drivers. Here we report SpaceBar, which enables simultaneous clone tracing and spatial gene expression profiling with standard imaging-based spatial transcriptomics pipelines.

Keywords single-cell biology virology lineage tracing COVID-19 SARS-CoV-2 influenza A virus-host interactions computational biology Get full text access Log in, subscribe or purchase for full access. References 1. Ravindra, N.G. ∙ Alfajaro, M.M. ∙ Gasque, V. ... Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes PLoS Biol. 2021; 19, e3001143 2. Melms, J.C. ∙ Biermann, J. ∙ Huang, H. ...