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Charelle Boot

As seen in: Nature, Science Magazine, Cell Reports
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Articles

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By Joep Beumer, Jens Puschhof, Fjodor Yousef Yengej, Adriana Martínez-Silgado, Marloes Blotenburg, Harry Begthel, Charelle Boot, Alexander van Oudenaarden, Ye-Guang Chen, Hans Clevers, Lianzheng Zhao
| Cell Reports Verified
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High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells

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By Joep Beumer, Jens Puschhof, Julia Bauzá-Martinez, Rasa Elmentaite, Kylie R. James, Alexander Ross, Delilah Hendriks, Benedetta Artegiani, Bas Ponsioen, Aurelia Saftien, Charelle Boot, Adriana Martínez-Silgado, Georg A. Busslinger, Amanda Andersson-Rolf
| ScienceDirect Verified
Highlights • A human organoid biobank combines hormone labeling and enteroendocrine cell generation • Transcriptomic profiling of human enteroendocrine cells uncovers differences with mice • Functional validation of EEC receptors and transcription factors • Secretome analysis reveals the repertoire of enteroendocrine secreted products Summary Enteroendocrine cells (EECs) sense intestinal content and release hormones to regulate gastrointestinal activity, systemic metabolism, and food intake.
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Prevention of tuberculosis infection and disease by local BCG in repeatedly exposed rhesus macaques

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By Sam Hofman, Karin Dijkman, Claudia C. Sombroek, Charelle Boot
| Nature Verified
Abstract Tuberculosis (TB) remains the deadliest infectious disease1, and the widely used Bacillus Calmette–Guérin (BCG) vaccine fails to curb the epidemic. An improved vaccination strategy could provide a cost-effective intervention to break the transmission cycle and prevent antimicrobial resistance2,3. Limited knowledge of the host responses critically involved in protective immunity hampers the development of improved TB vaccination regimens.
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