Ching-Ying Huang

1 Introduction Long-term fossil fuel usage has led to numerous problems ‎ [1-3]. Consequently, increasing research is being conducted into renewable and clean energy sources. Hydrogen energy has emerged as a suitable form of renewable energy. Being a clean and renewable source of energy, hydrogen plays a crucial role in decarbonization.

Abstract Most respiratory microbiome studies use amplicon sequencing due to high host DNA. Metagenomics sequencing offers finer taxonomic resolution, phage assessment, and functional characterization. We evaluated five host DNA depletion methods on frozen nasal swabs from healthy adults, sputum from people with cystic fibrosis (pwCF), and bronchoalveolar lavage (BAL) from critically ill patients. Median sequencing depth was 76.4 million reads per sample.