Eitan S. Kaplan

To examine the mutational burden of somatic variants in cancer driver genes in AD, we designed a hybrid capture gene panel covering 149 cancer driver genes with unique molecular identifier (UMI) barcoding (Table S1). We sequenced DNA from the prefrontal cortex (PFC) samples of 190 AD patients and 121 matched controls from the Religious Orders Study/Memory and Aging Project (ROSMAP) cohort at an average sequencing depth of >1,000× after UMI collapsing (Figures 1A, S1A, and S1B; Table S2).

Abstract Alzheimer's disease (AD) is the most common cause of dementia in older adults. Neuropathological and imaging studies have demonstrated a progressive and stereotyped accumulation of protein aggregates, but the underlying molecular and cellular mechanisms driving AD progression and vulnerable cell populations affected by disease remain coarsely understood.