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ArticleVolume 44, Issue 9p1799-1814.e11Open access Affiliations & Notes 1Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas MD Anderson Cancer Center, Houston, TX, United States of America 2Institute for Cell Therapy Discovery and Innovation, The University of Texas MD Anderson Cancer Center, Houston, TX, United States of America 3UTHealth Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center, Houston, TX, United States of...
Cloning of viral constructs We obtained the pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP73 from Sarah Teichmann (Addgene plasmid # 102796) and modified it to express a specific sgRNA using standard molecular cloning techniques.
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