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Nadima Uprety

As seen in: Cell Press, Cell Reports
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Articles

Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency

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By Alexander Biederstädt, Rafet Basar, Jeong-Min Park, Nadima Uprety, Rejeena Shrestha, Francia Silva, Merve Dede, John Watts, Sunil P Acharya, Donghai Xiong, Bin Liu, May Daher, Hind Rafei, Pinaki P. Banerjee, Ping Li, Sanjida Islam, Huihui Fan, Mayra Shanley, Jingling Jin, Bijender Kumar, Paul Lin, Silvia Tiberti, Xin Jiang, Patrick Zhang, Seema Rawal, Enli Liu, Luis Muniz-Feliciano, Elizabeth J. Shpall, Natalie Fowlkes, Ken Chen, Katayoun Rezvani, Vernikka Woods, Inci Biederstädt, Gary M. Deyter
| Cell Press Verified
Cloning of viral constructs We obtained the pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP73 from Sarah Teichmann (Addgene plasmid # 102796) and modified it to express a specific sgRNA using standard molecular cloning techniques.
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Generation of glucocorticoid-resistant SARS-CoV-2 T cells for adoptive cell therapy

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By Rafet Basar, Nadima Uprety, Emily Ensley, May Daher
| Cell Reports Verified
Highlights • Expansion of SARS-CoV-2 T cells from COVID19-recovered donors is feasible • The choice of cytokines modulates the phenotype and function of SARS-CoV-2 T cells • SARS-CoV-2 T cells are predominantly CD4+ and skewed to the Spike RBD region • NR3C1 KO renders SARS-CoV-2 T cells steroid resistant without functional alteration Summary Adoptive cell therapy with virus-specific T cells has been used successfully to treat life-threatening viral infections, supporting application of this...
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Generation of glucocorticoid resistant SARS-CoV-2 T-cells for adoptive cell therapy

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By Rafet Basar, Nadima Uprety, Emily Ensley, May Daher
| BioRxiv
Generation of glucocorticoid resistant SARS-CoV-2 T-cells for adoptive cell therapy Rafet Basar, Nadima Uprety, Emily Ensley, May Daher, Kimberly Klein, Fernando Martinez, Fleur Aung, Mayra Shanley, Bingqian Hu, Elif Gokdemir, Mayela Mendt, Francia Reyes Silva, Sunil Acharya, Tamara Laskowski, Luis Muniz-Feliciano, Pinaki Banerjee, Ye Li, Sufang Li, Luciana Melo Garcia, Paul Lin, Hila Shaim, Sean G Yates, David Marin, Indreshpal Kaur, Sheetal Rao, Duncan Mak, Angelique Lin, Qi Miao, Jinzhuang...
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