Flora Yang

Abstract The objective of the study was to evaluate the prevalence of pathogenic variants in 18 cancer predisposition genes in a national cohort of women with breast cancer diagnosed at age 40 or younger. Women with breast cancer at age ≤40 years were recruited from 33 Canadian centres at diagnosis. Germline genetic testing was performed for 18 genes (ATM, BARD1, BRCA1, BRCA2, BRIP1, CDH1, CHEK2, PALB2, PTEN, STK11, RAD51D, RAD51C, EPCAM, MLH1, MSH2, MSH6, and PMS2).

Abbreviations ACC, acetyl-CoA carboxylase ACP, acyl carrier protein LCMS, liquid chromatography coupled with mass spectrometry LPA, lysophosphatidic acid MOPS, 3-(morpholine-4-yl)propane-1-sulfonic acid PA, phosphatidic acid PE, phosphatidylethanolamine PG, phosphatidylglycerol ppGpp, guanosine tetraphosphate PS, phosphatidylserine The synthesis of lipids for membrane biogenesis consumes considerable amounts of energy and metabolites.

Abstract Exogenous fatty acids are directly incorporated into bacterial membranes, heavily influencing bacterial ecology and antibiotic susceptibility. We use liquid chromatography/mass spectrometry to characterize how exogenous fatty acids impact the Escherichia coli fatty acid synthesis pathway. We find that acyl-CoA synthesized from exogenous fatty acids rapidly increases long-chain acyl-ACP levels while depleting malonyl-ACP, indicating inhibition of fatty acid synthesis.