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ArticleVolume 38, Issue 8p1599-1617.e8Open access Affiliations & Notes 1University of Michigan, Department of Molecular & Integrative Physiology, Ann Arbor, MI, United States of America 2University of Michigan, Department of Biological Chemistry, Ann Arbor, MI, United States of America 3University of Michigan, Program in Cellular and Molecular Biology, Ann Arbor, MI, United States of America 4Medical Scientist Training Program, University of Michigan, Ann Arbor, MI, 48109, United States of...
Results To visualize ERMCSs in living cells, we generated eleven isogenic cell lines expressing a doxycycline-inducible split-GFP-based ERMCS reporter (SPLICS) together with a mitochondrial matrix red fluorescent protein reporter (mitoTagRFP)14,15,16,17,18 (Figure 1A). Selected clones exhibited inducible and reversible GFP fluorescence with stable RFP expression. Reporter induction did not affect cell growth, oxygen consumption, or extracellular acidification (Figures S1A–S1C).
Quantity GENERAL ADMISSION: $25.00 +$5.85 fee Quantity only 10 left! PREMIUM SEATING: $35.00 +$6.72 fee Table seating close to the stage. Must arrive 30 minutes before showtime. Note: This event is at our Midtown location *Promo Codes can be added in next step Note: This event is at our Midtown location
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