Hiroshi Ezura

1Plant Resilience Institute, Michigan State University, East Lansing, MI, USA 2Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA 3Department of Plant Biology, Michigan State University, East Lansing, MI, USA 4Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, USA 5Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska–Lincoln, Lincoln, NE, USA 6Instituto de...

Abstract We developed a non-culture and DNA-free genome editing technique for melon using in plant Particle Bombardment (iPB-RNP). The method was employed to create CmACO1 mutants. One of the mutant lines exhibited an extended shelf life due to ethylene deficiency during fruit ripening. As no cell culture step is involved, the iPB-RNP method is expected to overcome limitations associated with conventional genome editing, such as genotype dependency and somatic variations.

Abstract Volatiles from herbivore-infested plants function as a chemical warning of future herbivory for neighboring plants. (Z)-3-Hexenol emitted from tomato plants infested by common cutworms is taken up by uninfested plants and converted to (Z)-3-hexenyl β-vicianoside (HexVic). Here we show that a wild tomato species (Solanum pennellii) shows limited HexVic accumulation compared to a domesticated tomato species (Solanum lycopersicum) after (Z)-3-hexenol exposure.