Methods C57BL/6 or RAG1KO (B6.128S7-Rag1tm1.Mom/J) adult (6–8 weeks old) mice were sourced from the Animal Resource Centre (Perth, Australia) or the University of Queensland Biological Research Facility (UQBRF) at the Translational Research Institute (Brisbane, Australia). All mice were bred and housed under specific pathogen free (SPF) conditions at the UQBRF. Mice were regularly monitored for signs of distress and any adverse events were reported immediately to the ethics committee.