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Articles by Ian Slaymaker
High-resolution structure of cas13b and biochemical characterization of RNA targeting and cleavage
(Cell Reports 26, 3741–3751.e1–e5; March 26, 2019) In our paper, we reported the structure and biochemical characterization of the RNA-guided RNase Cas13b from Prevotella buccae (PbuCas13b). This work included an assessment of the turnover rate of this enzyme. Recently, it was brought to our attention by Juan Santiago and Ashwin Ramachandran that the reported turnover rate was inconsistent with our data (Figure 5D). Upon re-evaluation of our raw data, we found an error in the units.
High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage
Highlights • We report the crystal structure of PbuCas13b at 1.65 Å resolution • We present and validate models for RNA targeting and cleavage • We rationally engineer Cas13b to change RNA cleavage specificity • Cas13b is structurally unrelated to other CRISPR systems Summary Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members.
If CRISPR is so amazing, why can't we edit human genes yet?
Despite all the promise, human gene editing is yet to reach the clinic Few techniques have shaken science quite like CRISPR. The quick, easy and cheap gene editing tool was hailed as the "breakthrough of 2015" by Science magazine. It has the potential to change entire family lineages, treating disorders from cancer to HIV. Yet five years after bursting onto the scene, CRISPR remains notably absent from clinics.
Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits
CHD8 Is Expressed in Most Cell Types throughout the Brain CHD8 Affects Pathways Involved in Cell Cycle and Histone and Chromatin Modification Cortical Lamination, Major Cell Types, and Late-Stage Cortical Progenitor Number in the Somatosensory Cortex Do Not Vary between Chd8+/– and Wild-Type Mice Chd8+/– Mice Exhibit Broad Gene Expression Changes throughout the Brain Chd8+/– Mice Exhibit Synaptic Dysfunction within MSNs in the NAc CHD8 Is Expressed in Most Cell Types throughout the Brain CHD8...
Broad, MIT scientists overcome key CRISPR-Cas9 genome editing hurdle
Researchers at the Broad Institute of MIT and Harvard and the McGovern Institute for Brain Research at MIT have engineered changes to the revolutionary CRISPR-Cas9 genome editing system that significantly cut down on "off-target" editing errors. The refined technique addresses one of the major technical issues in the use of genome editing. The CRISPR-Cas9 system works by making a precisely targeted modification in a cell's DNA.
Rationally engineered Cas9 nucleases with improved specificity
The RNA-guided endonuclease Cas9 is a versatile genome editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing.
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