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J. David Barrass on Muck Rack

J. David Barrass

As seen in: Cell Press, JoVE- Journal of Visualized Experiments
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Articles

Peer Reviewed Scientific Video Journal - Methods and Protocols

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By J. David Barrass
| JoVE- Journal of Visualized Experiments
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Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

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By J. David Barrass
| JoVE- Journal of Visualized Experiments
Biochemistry Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help. An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help. Summary The use of thiolated uracil to sensitively and specifically purify newly transcribed RNA from the yeast Saccharomyces cerevisiae.
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Tuning Degradation to Achieve Specific and Efficient Protein Depletion

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By J. David Barrass, Gonzalo I. Mendoza-Ochoa, Isabella E. Maudlin, Emanuela Sani
| JoVE- Journal of Visualized Experiments
Immunology and Infection Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help. An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help. Summary Here, we present a protocol to effectively and specifically deplete a protein of interest in the yeast Saccharomyces cerevisiae using the β-est AID system.
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