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Jahkwaun Budhai on Muck Rack

Jahkwaun Budhai

As seen in: Cell Press
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Articles

Environmentally induced cell stiffening shapes metastatic site selection by tuning the immune vulnerability of cancer cells

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By Yassmin Elbanna, Maria Tello-Lafoz, Ye Zhang, Chengyuan Li, Seema Nusrat Amin, Zhenghan Wang, Jun-Goo Kwak, Alexandrina M Yakimov, Esther Lee, Sarah Duquette, Young Kim, Jahkwaun Budhai, Ning Fan, Erik Ladewig, Tejus A. Bale, Benjamin Y. Winer, Kenny Yu, Joan Massagué, Christina Leslie, Jungwoo Lee, Ori Barzilai, Scott R. Manalis, Morgan Huse, Aliya Holland, Anastasiia Vaska, Myra Dada, Samuel Vayner
| Cell Press Verified
Cytotoxic lymphocyte preparation To generate OT-1 CTLs, splenocytes from OT-1 αβTCR transgenic mice were mixed with congenic splenocytes pulsed with 100 nM OVA and cultured in RPMI medium with 10% FBS and 0.55 mM β-mercaptoethanol. After 24 hours, cells were supplemented with 30 IU/mL IL-2 (NIH BRB Repository) and split as needed. Functional assays were performed after 7 days in culture.
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