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Environmentally induced cell stiffening shapes metastatic site selection by tuning the immune vulnerability of cancer cells
Cytotoxic lymphocyte preparation To generate OT-1 CTLs, splenocytes from OT-1 αβTCR transgenic mice were mixed with congenic splenocytes pulsed with 100 nM OVA and cultured in RPMI medium with 10% FBS and 0.55 mM β-mercaptoethanol. After 24 hours, cells were supplemented with 30 IU/mL IL-2 (NIH BRB Repository) and split as needed. Functional assays were performed after 7 days in culture.
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