1 Introduction Hepatitis B virus (HBV) DNA is an important biomarker in the management of chronic hepatitis B (CHB), as it reflects viral replication, guides treatment decisions, and predicts long-term risks of liver-related complications [1]. Real-time polymerase chain reaction (PCR) is the current standard for serum HBV DNA detection, with multiple commercial assays available. Despite the widespread use of real-time PCR, it is not without limitations.