James Walshe

Abstract H3K36me3 is a hallmark of actively and recently transcribed genes and contributes to cellular memory and identity. The deposition of H3K36me3 occurs co-transcriptionally when the methyltransferase SETD2 associates with RNA polymerase II. Here we present three cryo-EM structures of SETD2 bound to RNA polymerase II elongation complexes at different states of nucleosome passage.

Abstract The protein IWS1 (Interacts with SPT6 1) is implicated in transcription-associated processes, but a direct role in RNA polymerase (Pol) II function is unknown. Here, we use multi-omics kinetic analysis after rapid depletion of IWS1 in human cells to show that loss of IWS1 results in a global decrease of RNA synthesis and a global reduction in Pol II elongation velocity.

We present a case report describing a rare cause of a common obstetric complication; pyloric web causing gross polyhydramnios. Polyhydramnios affects 1–2 % of pregnancies, and a proportion of these have an associated fetal anomaly that is not detectable antenatally. Pyloric webs are a rare cause of neonatal gastric outflow obstruction, however the exact incidence is unclear, and has been reported as less than 1 per million live births [ 1 2 ]. Treatment is by surgical excision.