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Science & SocietyOnline nowOpen access 1Swiss Bee Research Centre, Agroscope, Berne 3003, Switzerland 2Institute of Bee Health, Vetsuisse Faculty, University of Bern, Liebefeld, 3097, Switzerland 3School of Agriculture, Food, and Wine, University of Adelaide, Adelaide, SA 5064, Australia 4Plant Ecology and Nature Conservation Group, Wageningen University, Wageningen, 6708, The Netherlands 5School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada 6Centre for...
In vitro larval inoculation assays To test whether TGIP can be triggered experimentally, honey bee larvae originating from healthy colonies were reared in vitro before the queen was exposed to the bacterium to assess the basal mortality induced by M. plutonius inoculation. Mortality was measured again after the exposure of the queen to assess the protective effect of this exposure on the brood (Fig 1a).
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