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3D FRP Reinforcement Systems for Concrete Beams: Innovation towards High Performance Concrete Structures
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A programmable targeted protein-degradation platform for versatile applications in mammalian cells and mice
Highlights • Screening for truncation variants of Trim21 with enhanced degradation efficiency • Developing a RedΔTrim-TPD system for the inhibition of HSV-1 virus • Developing a ChemΔ2Trim-TPD system for Cas9/dCas9 control in gene editing • Creating an OptoΔ2Trim-TPD system to inhibit tumor growth in mice Summary Myriad physiological and pathogenic processes are governed by protein levels and modifications.
A programmable targeted protein-degradation platform for versatile applications in mammalian cells and mice
Myriad physiological and pathogenic processes are governed by protein levels and modifications. Controlled protein activity perturbation is essential to studying protein function in cells and animals. Based on Trim-Away technology, we screened for truncation variants of E3 ubiquitinase Trim21 with elevated efficiency (ΔTrim21) and developed multiple ΔTrim21-based targeted protein-degradation systems (ΔTrim-TPD) that can be transfected into host cells.
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