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KEVIN WASKO on Muck Rack

KEVIN WASKO

(He/Him)
Toronto
As seen in: BioRxiv, Cell Press
Covers:  Health Policy, Canadian Politics
(he/him) Emergency Medicine Physician. Views are my own.

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KEVIN WASKO’s Biography

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Dr. Kevin Wasko is a practicing emergency physician with Trillium Health
Partners in Mississauga, ON. He was previously the Physician Executive for
Integrated Rural Health with the Saskatchewan Health Authority and was jointly responsible for delivering health care services across rural Saskatchewan. Dr. Wasko was the physician co-lead on the Saskatchewan Health System Transition Team in 2017, leading work in the areas of physician engagement, medical governance and the academic mandate. During…

Articles

Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing

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By Honglue Shi, Noor Al-Sayyad, KEVIN WASKO, Marena Trinidad, Erin Doherty Verified, Kamakshi Vohra, Ron S Boger, David Colognori, Joshua C. Cofsky, Petr Skopintsev, Zev Bryant, Jennifer Doudna
| Cell Press Verified
Keywords genome editing CRISPR-Cas9 protospacer-adjacent motifs target search DNA recognition DNA unwinding biochemistry biophysics single-molecule manipulation kinetic models Introduction The widespread utility of CRISPR-Cas9 for programmable genome editing in human cells, plants, and other eukaryotes has propelled interest in understanding the determinants of efficient genome modification.1,2 Cas9 recognizes target sequences within genomes using an ATP-independent process in which...
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Directed evolution expands CRISPR-Cas12a genome editing capacity

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By Enbo Ma, Kai Chen, Honglue Shi, KEVIN WASKO
| BioRxiv
Abstract CRISPR-Cas12a enzymes are versatile RNA-guided genome-editing tools with applications encompassing viral diagnosis, agriculture and human therapeutics. However, their dependence on a 5'-TTTV-3' protospacer-adjacent motif (PAM) next to DNA target sequences restricts Cas12a's gene targeting capability to only ~1% of a typical genome.
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Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing

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By Hannah Karp, Madeline A. Zoltek, KEVIN WASKO, Angel Vazquez
| BioRxiv
Abstract Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing and cytotoxicity. Clinical use of Cas9 RNPs currently entails electroporation into cells ex vivo, but no systematic comparison of this method to packaged RNP delivery has been made.
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