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ArticleVolume 7, Issue 1102549Open access 1Center for Cancer Cell Therapy, Stanford Cancer Institute, Stanford University, Stanford, CA, USA 2Department I of Internal Medicine, Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf, University of Cologne, Medical Faculty and University Hospital Cologne, Cologne, Germany 3Cancer Center Cologne Essen (CCCE), Cologne, Germany 4Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany 5University of Cologne,...
Nalm6-GD2, CHLA255, SY5Y and SMS-SAN tumor cell lines were cultivated in RPMI (Gibco) supplemented with 10% FBS (Sigma Aldrich) and 1% Penicillin/Streptomycin (Gibco). For in vivo studies SY5Y tumor cells were resuspended at 5 × 106/ml in PBS and 200 µl (= 1 × 106 tumor cells) were injected via tail vein injection into six- to ten-week-old female NOD-SCID γc−/− (NSG) mice. Mice were bred in house under Stanford University APLAC-approved protocols as described previously [41].
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