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Makhosazana Dlamini on Muck Rack

Makhosazana Dlamini

As seen in: Nature, Frontiers in Public Health, MDPI, IOL News, Business Report, Pretoria News, Sunday Tribune, persfin.co.za, tios.co.za
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Articles

Targeted next-generation sequencing implementation in Eswatini identifies rifampicin and bedaquiline resistance undetected by routine diagnostic testing

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By Debrah Vambe, Alexander Kay, Mangaliso Ziyane, Sein Sein Thi, Leonardo De Araujo, Viola Dreyer, Tanja Niemann Verified, Andrea Maurizio Cabibbe, Jennifer Furin, Lindokuhle Dlamini, Gugu Maphalala, Makhosazana Dlamini, Daniela Maria Cirillo, Anna M Mandalakas, Christoph Lange, Christian Utpatel, Sindisiwe Dlamini, Stefan Niemann Verified, Mdigo Thunzini, Busizwe Sibandze, Sijabu Masina, Tafadzwa Mazuruse, Thulani Jele, Bheki Mamba, Mbongeni Dube, Nomthandazo Lukhele, Sphiwe Ngwenya
| Nature Verified
Abstract In Eswatini, multidrug-resistant (MDR) Mycobacterium tuberculosis (Mtb) strains harbouring the rifampicin-resistance (RR) rpoB I491F mutation are missed by routine diagnostics, including GeneXpert MTB/RIF Ultra (Xpert Ultra), line probe assays (LPA), and mycobacterium growth indicator tube (MGIT) phenotypic drug susceptibility testing (pDST). To address this diagnostic gap, Eswatini introduced targeted next-generation sequencing (tNGS) in 2019.
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Advanced HIV disease among newly diagnosed clients identified through community- and facility-based HIV testing services in Eswatini: a cross-sectional study

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By Samson M. Haumba, Makhosazana Dlamini, Sylvia Ojoo, Thokozani Maseko, Fezokuhle Khumalo, Nakekelo Mndzebele
| Frontiers in Public Health Verified
Abstract Background: Despite the Kingdom of Eswatini surpassing the UNAIDS 95–95-95 targets, the persistence of Advanced HIV Disease (AHD) remains a clinical concern. Advanced HIV Disease increases morbidity and mortality. This study evaluated the rate of AHD among newly diagnosed clients aged ≥15 identified through the community-based testing in comparison to the facility-based testing.
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Conserved neutrophil degranulation transcripts in HIV-TB coinfected children across East and Southern Africa

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By Eric Katagirya, Busisiwe C. Mlotshwa, Samuel Kyobe, Savannah Mwesigwa, Gaone Retshabile, Lesedi Williams, Marion Amujal, John Mukisa, Gerald Mboowa, David P Kateete, Misaki Wayengera, Sununguko Wata Mpoloka, Ishmael Kasvosve, Makhosazana Dlamini, Adeodata R Kekitiinwa, Moses Joloba, Eddie Wampande, Chester Brown, Graeme Mardon, Neil A. Hanchard, Mogomotsi Matshaba, Angella N. Mirembe, Koketso Morapedi, Betty Nsangi, Grace P. Kisitu, Gabriel Anabwani, Dithan Kiragga, Florence Anabwani-Richter
| Nature Verified
Abstract HIV-tuberculosis (HIV-TB) coinfection poses a significant public health challenge among children in high-burden African regions. Most previous transcriptomic studies have concentrated on adults and non-African populations, primarily analyzing gene-level differential expression. This approach overlooks multi-isoform complexity and may obscure both inherent and pathogen-induced intragenic heterogeneity.
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