Mara Barucco

Yeast strains All experiments were performed using Saccharomyces cerevisiae strains of the W303 background. Strains were grown at 30 °C according to classical procedures in standard yeast extract peptone dextrose (YPD) or dropout base supplemented with the appropriate CSM-amino acid (MP Biomedicals, LCC) media. To induce ER stress, yeast cells were treated with 2 mM of dithiothreitol (DTT, VWR) or 1 μg/ml of tunicamycin (Tm, Sigma).

Highlights • Degradation of ncRNAs by the exosome frees bound termination factors Nab3 and Nrd1 • Exosome inhibition triggers accumulation of ncRNAs complexed with Nab3 and Nrd1 • Out-titration of Nab3 and Nrd1 by ncRNAs decreases NNS co-transcriptional binding • A circular RNA decoy recapitulates the global impairment of NNS-dependent termination Summary A large share of the non-coding transcriptome in yeast is controlled by the Nrd1-Nab3-Sen1 (NNS) complex, which promotes transcription...