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Articles by Max J. Kellner
Tumor sialylation regulates G-CSF stability and promotes neutrophil-mediated immunosuppression in breast cancer
Abstract Breast cancer is the most prevalent cancer among women, yet immune checkpoint inhibitors remain largely ineffective in the majority of patients. Here we show that tumor sialylation is negatively associated with T cell infiltration across molecular breast cancer subtypes.
A split-site E3 ligase mechanism enables ZNFX1 to ubiquitinate and cluster single-stranded RNA into ubiquitin-coated nucleoprotein particles
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Keywords innate immunity E3 ubiquitin ligase helicase ZNFX1 nucleic acid sensor non-canonical ubiquitination Introduction Innate immunity targets microbial invaders via pathogen-associated molecular patterns (PAMPs).1 Viruses are typically recognized by the presence of nucleic acids (NAs) in incorrect cellular compartments.
By Daniel B. Grabarczyk, Eric Aird, Paul C. Kirchgatterer, Julian F. Ehrmann, Robert Kurzbauer, Lillie Bell, Max J. Kellner, Ritika Aggarwal, Alexander Schleiffer, Anton Meinhart, Gijs A. Versteeg, Josef M Penninger, Lukas S. Stelzl, Moritz M. Gaidt, Ingrid Tessmer, Jacob Corn, Tim Clausen, Vanessa Reznikow, Victoria Faas, Luiza Deszcz
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Cell Press
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Bat organoids reveal antiviral responses at epithelial surfaces - Nature Immunology
Abstract Bats can host viruses of pandemic concern without developing disease. The mechanisms underlying their exceptional resilience to viral infections are largely unresolved, necessitating the development of physiologically relevant and genetically tractable research models.
Angiotensin-converting enzyme 2-at the heart of the COVID-19 pandemic
Hoffmann M. Kleine-Weber H. Schroeder S. Kruger N. Herrler T. Erichsen S. Schiergens T.S. Herrler G. Wu N.H. Nitsche A. et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020; 181: 271-280.e278https://doi.org/10.1016/j.cell.2020.02.052 Donoghue M. Hsieh F. Baronas E. Godbout K. Gosselin M. Stagliano N. Donovan M. Woolf B. Robison K. Jeyaseelan R. et al.
High-resolution structure of cas13b and biochemical characterization of RNA targeting and cleavage
(Cell Reports 26, 3741–3751.e1–e5; March 26, 2019) In our paper, we reported the structure and biochemical characterization of the RNA-guided RNase Cas13b from Prevotella buccae (PbuCas13b). This work included an assessment of the turnover rate of this enzyme. Recently, it was brought to our attention by Juan Santiago and Ashwin Ramachandran that the reported turnover rate was inconsistent with our data (Figure 5D). Upon re-evaluation of our raw data, we found an error in the units.
Scalable, rapid and highly sensitive isothermal detection of SARS-CoV-2 for laboratory and home testing
New Results Max J Kellner, James J Ross, Jakob Schnabl, Marcus P.S. Dekens, Robert Heinen, Nathan A Tanner, Robert Fritsche-Polanz, Marianna Traugott, Tamara Seitz, Alexander Zoufaly, Manuela Foedinger, Christoph Wenisch, Johannes Zuber, Vienna Covid-19 Diagnostics Initiative (VCDI), View ORCID ProfileAndrea Pauli, Julius Brennecke doi: https://doi.org/10.1101/2020.06.23.166397 Abstract Global efforts to monitor and contain the Covid-19 pandemic, caused by the beta-coronavirus SARS-CoV-2,...
SHERLOCK: Nucleic acid detection with CRISPR nucleases
Abstract Rapid detection of nucleic acids is integral for applications in clinical diagnostics and biotechnology. We have recently established a CRISPR-based diagnostic platform that combines nucleic acid pre-amplification with CRISPR-Cas enzymology for specific recognition of desired DNA or RNA sequences.
Author Correction: SHERLOCK: nucleic acid detection with CRISPR nucleases
Max J. Kellner1, Jeremy G. Koob1, Jonathan S. Gootenberg1,2,3, Omar O. Abudayyeh1,2,3 & Feng Zhang1,2,3,4 Nature Protocols (2020)Cite this article The original article was published on 23 September 2019 Correction to: Nature Protocols https://doi.org/10.1038/s41596-019-0210-2, published online 23 September 2019. In the version of this article initially published, three of the sensitivity values in the bottom table in Box 1 were incorrect.
SHERLOCK: nucleic acid detection with CRISPR nucleases
Abstract Rapid detection of nucleic acids is integral to applications in clinical diagnostics and biotechnology. We have recently established a CRISPR-based diagnostic platform that combines nucleic acid pre-amplification with CRISPR–Cas enzymology for specific recognition of desired DNA or RNA sequences.
Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13
Omar O. Abudayyeh, PhD, 43 Vassar St. Cambridge, MA 02139, E-mail Address: omarabu@mit.edu; Broad Institute of MIT and Harvard, Cambridge, Massachusetts; Harvard Medical School, Boston, Massachusetts. McGovern Institute for Brain Research at MIT, Cambridge, Massachusetts; Departments of Harvard Medical School, Boston, Massachusetts. Brain and Cognitive Science, Harvard Medical School, Boston, Massachusetts. Biological Engineering, and Harvard Medical School, Boston, Massachusetts.
High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage
Highlights • We report the crystal structure of PbuCas13b at 1.65 Å resolution • We present and validate models for RNA targeting and cleavage • We rationally engineer Cas13b to change RNA cleavage specificity • Cas13b is structurally unrelated to other CRISPR systems Summary Type VI CRISPR-Cas systems contain programmable single-effector RNA-guided RNases, including Cas13b, one of the four known family members.
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al.
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
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RNA editing with CRISPR-Cas13
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RNA editing with CRISPR-Cas13
Nucleic acid editing holds promise for treating genetic disease, particularly at the RNA level, where disease-relevant sequences can be rescued to yield functional protein products. Type VI CRISPR-Cas systems contain the programmable single-effector RNA-guided RNases Cas13.
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