Xianying Zhang

To determine whether α-Syn-6H-EGFP could be used to explore the pathological effects of α-Syn inclusions on transcription, we performed scRNA-seq on SNc dopaminergic neurons. Single neurons were collected using the patch-seq approach74 (Figure 5A). Neurons with α-Syn-6H-EGFP inclusions were identified by their filamentous structures and enhanced fluorescence (Figure S5A).

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(A) Schematic diagram showing two AAV-helpers and rabies virus (left) injected into DVC of Tac1-IRES-Cre mice (right) to map monosynaptic inputs of Tac1+ DVC neurons. (B) Timeline for injection of AAV-helpers and rabies virus. (C) Merged and single-channel example micrographs showing starter cells in DVC. (D) Example coronal sections showing distribution of RV-labeled EGFP+ cells in CeA (D1), BNST (D2), PVH (D3), PSTh (D4), and brain areas in pons and medulla of hindbrain (D5).