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Metabolite Profiling

As seen in: ACS Publications, Cell Press
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Articles

Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling

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By Wondong Kim, Amy Deik, Metabolite Profiling, Clicerio Gonzalez
| Cell Press Verified
Highlights • Blocking ETC or lactate production reduces cytosolic NAD +/NADH and increases HUFAs • HUFA synthesis by D5D and D6D is a mechanism for glycolytic NAD + recycling • D5D- and D6D-mediated NAD + regeneration can be acutely adaptive in vivo • SLC16A11 and FADS1-3 may influence metabolic risk via impact on cytosolic NAD +/NADH Summary The reactions catalyzed by the delta-5 and delta-6 desaturases (D5D/D6D), key enzymes responsible for highly unsaturated fatty acid (HUFA) synthesis,...
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Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte

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By Metabolite Profiling, Piia Liigand, Karl Kaupmees, Kristjan Haav
| ACS Publications
Mass spectrometry (MS) with electrospray ionization (ESI (1)) source is a key technique in various research fields, ranging from food and environmental analysis to metabolomics and proteomics. (2) However, despite its widespread application, current understanding of the ESI process is still limited. (3, 4) Ionization efficiency (IE), the amount of ions generated from a specific compound in the ionization source, may vary from compound to compound by more than 6 orders of magnitude.
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