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LRRC58 defines an E3 ubiquitin ligase complex sensitive to cysteine abundance
STAR★Methods REAGENT or RESOURCESOURCEIDENTIFIER Antibodies rabbit anti-CDO1 Proteintech Cat# 12589-1-AP; RRID: AB_10638145 rabbit anti-GFP Abcam Cat# ab290; RRID: AB_2313768 mouse anti-FLAG (M2) Merck Cat# F3165; RRID: AB_259529 rabbit anti-HA (C29F4) Cell Signaling Cat# 3724; RRID: AB_1549585 mouse anti-Ubiquitin Life Sensors Cat# VU101; RRID: AB_2716558 rabbit anti-Cul2 Bethyl Laboratories Cat# A302-476A; RRID: AB_1944215 rabbit anti-Cul5 Bethyl Laboratories Cat# A302-173A; RRID:...
Ageing versus developmental silencing: Answers from the epigenome
DNA deoxyribonucleic acid H3K27me3 histone 3 lysine 27 trimethylation H3K9me2 histone 3 lysine 9 dimethylation H3K9me3 histone 3 lysine 9 trimethylation RNA ribonucleic acid TSSs transcription start sites Introduction During early development as cell transition from multipotency to committed cell fates, the chromatin remains accessible so that cells can respond to differentiation cues and activate genes required for differentiation and acquisition of identity.
Single-Cell Profiling and SCOPE-Seq Reveal Lineage Dynamics of Adult Ventricular-Subventricular Zone Neurogenesis and NOTUM as a Key Regulator
Highlights • Neurogenesis studied in ventricular-subventricular zone (V-SVZ) and olfactory bulb (OB) • scRNA-seq reveals lineage progression from V-SVZ to OB and a Notum+ NSC transition • SCOPE-seq identifies a Notum+ NSC intermediate based on cell size and NOTUM targets • NOTUM prevents uncontrolled expansion of neuronal progenitors in the V-SVZ Summary In the adult ventricular-subventricular zone (V-SVZ), neural stem cells (NSCs) generate new olfactory bulb (OB) neurons and glia throughout...
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