Simai Wang

Abstract Despite decades of biochemical and structural studies of the nucleosome1, researchers lack genome-scale methods to determine variability in nucleosome structure along individual chromatin fibres. To address this, here we present Iteratively Defined Lengths of Inaccessibility (IDLI), a computational method that maps the single-molecule co-occupancy of structurally distinct nucleosomes, subnucleosomes and other protein–DNA interactions through long-read single-molecule footprinting2,3.

Keywords transcription mRNA processing gene expression transcriptional elongation cellular senescence aging Get full text access Log in, subscribe or purchase for full access. References 1. Aoi, Y. ∙ Shilatifard, A. Transcriptional elongation control in developmental gene expression, aging, and disease Mol. Cell. 2023; 83:3972-3999 2. Aoi, Y. ∙ Smith, E.R. ∙ Shah, A.P. ... NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release Mol. Cell. 2020; 78:261-274.e5 3. Tufan, T.

Abstract We present a genome-scale method to map the single-molecule co-occupancy of structurally distinct nucleosomes, subnucleosomes, and other protein-DNA interactions via long-read high-resolution adenine methyltransferase footprinting.