Soman N. Abraham

Mouse infection UPEC CI5, UTI89, or NU14 was statically grown overnight in Luria-Bertani medium. Prior to inoculation, bacterial cultures were pelleted by centrifugation, washed once with sterile phosphate-buffered saline (PBS), and then resuspended in PBS. The optical density at 600 nm was measured to ensure a final concentration of 1 × 108 CFU per mouse. Anesthetized mice were transurethrally inoculated.

Abstract The critical events that trigger sepsis progression into life-threatening septic shock remain unclear. In agreement with reports that link a drop in platelet count to a complicated clinical course in sepsis patients, here we report that, during sepsis, mouse platelets become activated, deposit systemically on vascular walls, and stimulate perivascular mast cells (MC) by releasing platelet activating factor (PAF).

Spectrum of clinical phenotypes of PLCG2 gene variants: Just PLAID Supported in part by the David’s Dream Funds. Disclosure of potential conflict of interest: The author declares that he has no relevant conflicts of interest. Crown Copyright © 2024 Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology.