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Sung Ho Boo

As seen in: Cell Press, Cell Reports
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Articles

Ribonuclease κ promotes longevity by preventing age-associated accumulation of circular RNA in stress granules

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By Sieun Kim, Seokjin Ham, Sung Ho Boo, DongHun Lee, Hyemin Min, Eunseok Kang, Rosa Haque, Hanseul Lee, Yoonji Jung, Sujeong Kwon, Sangsoon Park, Hae-Eun H. Park, Eun Ji E. Kim, Wooseon Hwang, Eunah Kim, Gee-Yoon Lee, Kun-Young Park, Jae Myoung Suh, Gwangrog Lee, Yoon Ki Kim, Seung-Jae Lee
| Cell Press Verified
Keywords circular RNA ribonuclease κ stress granule aging senescence Caenorhabditis elegans Get full text access Log in, subscribe or purchase for full access. References 1. Cocquerelle, C. ∙ Mascrez, B. ∙ Hétuin, D. ... Mis-splicing yields circular RNA molecules FASEB J. 1993; 7:155-160 2. Liu, C.X. ∙ Chen, L.L. Circular RNAs: Characterization, cellular roles, and applications Cell. 2022; 185:2016-2034 3. Cortés-López, M. ∙ Gruner, M.R. ∙ Cooper, D.A. ...
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UPF1 promotes rapid degradation of m6A-containing RNAs

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By Sung Ho Boo, Hongseok Ha, Yujin Lee, Min-Kyung Shin
| Cell Reports Verified
Method details Plasmid construction ); pCMV-MYC-PNRC2 and pcDNA3-FLAG-UPF1 (); pcDNA3-FLAG, pcDNA3-FLAG-YTHDF2-FL, pcDNA3-FLAG-YTHDF2-N, pcDNA3-FLAG-YTHDF2-C, pMS2-HA, pMS2-HA-YTHDF2-N, pMS2-HA-YTHDF2(1-100), pMS2-HA-YTHDF2(101-200), pMS2-HA-YTHDF2-NΔ101-200, pMS2-HA-YTHDF2(201-383), pMS2-HA-PNRC2, pCMV-MYC-YTHDF1, pCMV-MYC-YTHDF2, pCMV-MYC-YTHDF3, and pcβ-6bs (); phCMV-MUP (); pCMV-MYC (Clontech); pCMV-MYC-UPF1 The following constructs have been described previously: pBG-SON-WT, pBG-SON-Mut,...
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Endoribonucleolytic Cleavage of m6A-Containing RNAs by RNase P/MRP Complex

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By Hongseok Ha, Yujin Lee, Ok Hyun Park, Sung Ho Boo
| Cell Press Verified
Highlights • m 6A-containing RNAs are degraded by an endoribonucleolytic cleavage pathway • A YTHDF2-HRSP12-RNase P/MRP axis contributes to m 6A-mediated RNA decay • An interaction between YTHDF2 and RNase P/MRP is bridged by HRSP12 • m 6A-containing circular RNAs are degraded by the YTHDF2-HRSP12-RNase P/MRP pathway Summary N 6-methyladenosine (m 6A) is the most abundant internal modification in RNAs and plays regulatory roles in a variety of biological and physiological processes.
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