Tianying Zhang
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Reoviridae -derived short trimerization domain for stabilizing homo-trimerization of vaccine immunogens and therapeutic proteins
Abstract Trimerization motifs play pivotal roles in structural biology and therapeutic protein engineering. Here, we describe a short trimerization motif (rFd1303) derived from the Reoviridae family reovirus σ1 protein. Compared with the widely used T4-Foldon, the rFd1303 increased recombinant protein yields. The rFd1303-fused immunogens of SARS-CoV-2 spike and influenza hemagglutinin elicited antibody responses comparable to T4-Foldon-fused controls in mice.
Integrative single-cell and bulk transcriptomic analysis identify R-loop-associated gene signatures driving immune-inflammatory networks in acute pancreatitis
Abstract Background: R-loop dysregulation has been implicated in chronic inflammation via DNA damage and cytoplasmic ssDNA release, yet its role in acute pancreatitis (AP) remains to be systematically investigated. This study aimed to identify R-loop-related biomarkers in AP and elucidate their potential roles in immune-inflammatory networks through integrative bioinformatics analysis and in vitro experimental validation.
Krüppel-like factor 2 programs early exhausted T cell states and restrains antiviral immunity
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Keywords CD8+ T cell chronic infection in vivo pooled CRISPR screening CX3CR1 KLF2 S1PR1 PD-1 immunotherapy TNF anti-viral effector-like cells T cell exhaustion immunopathology Get full text access Log in, subscribe or purchase for full access. References 1. Omilusik, K.D. ∙ Goldrath, A.W. Remembering to remember: T cell memory maintenance and plasticity Curr. Opin. Immunol. 2019; 58:89-97 2. Herndler-Brandstetter, D. ∙ Ishigame, H. ∙ Shinnakasu, R. ...
By Zifeng Li, Wenhao Li, Huiqing Liang, Fan Xu, Leilei Zhang, Wei Zhang, Yanhan Wang, Yurui Zhou, Shijie Luo, Xing Lei, Hailang Li, Wei-Feng Huang, Meixia Che, Wen-Hsien Liu, Jialiang Huang, Tianying Zhang, Kejia Wang, Nengming Xiao, Kairui Mao, Yajun Jiang, Hongling Huang, Shengjun Geng, Shengyong Tao
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Cell Press
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