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Articles

DMF-DM-seq: Digital-Microfluidics Enabled Dual-Modality Sequencing of Single-Cell mRNA and microRNA with High Integration, Sensitivity, and Automation

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By yingwen chen, Xuanqun Wang, Xing Na, Yingkun Zhang
| ACS Publications
Single-cell mRNA sequencing (scRNA-seq), a powerful tool for defining the cellular diversity in multicellular organisms by parsing differential gene expression, has been applied in a variety of studies. (1−4) However, the variation between single cells cannot be fully understood by mRNA profiling alone, since post-transcriptional regulation plays a pivotal role in shaping single-cell heterogeneity.
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Highly Multiplexed, Efficient, and Automated Single‐Cell MicroRNA Sequencing with Digital Microfluidics.

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By yingwen chen, Xuanqun Wang, Xing Na, Yingkun Zhang
| pericles.pericles-prod.literatumonline.com
Conflict of Interest The authors declare no conflict of interest Supporting Information Filename Description smtd202301250-sup-0001-SuppMat.pdf971.3 KB Supporting Information References 1, , , Nat. Rev. Genet. 2012, 13, 358. 2(a) , , , , , , , , , , , , Nat. Neurosci. 2018, 21, 1784; (b) , , , , , , Neuron 2012, 73, 35. 3(a) , , Front. Bioeng. Biotechnol. 2020, 8, 619583; (b) , , Nat. Rev. Mol. Cell Biol. 2014, 15, 565. 4(a) , , , , , , , , , , , , , , , Nat. Commun. 2019, 10, 95; (b) , , , , , , , Nat. Commun.
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mitoSplitter: A mitochondrial variants-based method for efficient demultiplexing of pooled single-cell RNA-seq

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By Xinrui Lin, yingwen chen, Li Lin, Kun Yin
| BioRxiv
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